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dc.contributor.authorHeikaus, Laura
dc.contributor.authorSchlüter, Hartmut
dc.contributor.authorNurul Hidayah, Siti
dc.contributor.authorGaikwad, Manasi
dc.date.accessioned2021-06-02T10:12:13Z
dc.date.available2021-06-02T10:12:13Z
dc.date.issued2019
dc.identifierONIX_20210602_10.5772/intechopen.89644_439
dc.identifier.urihttps://library.oapen.org/handle/20.500.12657/49325
dc.description.abstractA characteristic of many proteoforms, derived from a single gene, is their similarity regarding the composition of atoms, making their analysis very challenging. Many overexpressed recombinant proteins are strongly associated with this problem, especially recombinant therapeutic glycoproteins from large-scale productions. In contrast to small molecule drugs, which consist of a single defined molecule, therapeutic protein preparations are heterogenous mixtures of dozens or even hundreds of very similar species. With mass spectrometry, currently high-quality spectra of intact proteoforms can be obtained only, if the complexity of the mixture of individual proteoform-ions, entering the gas phase at the same time is low. Thus, prior to mass spectrometric analysis, an effective separation is required for getting fractions with a low number of individual proteoforms. This is especially true not only for recombinant therapeutic proteins, because of their huge heterogeneity, but also relevant for top-down proteomics. Purification of proteoforms is the bottleneck in analyzing intact proteoforms with mass spectrometry. This review is focusing on the current state of the art, especially of liquid chromatography for preparing proteoforms for mass spectrometric top-down analysis. The topic of therapeutic proteins has been chosen, because this group of proteins is most challenging regarding their proteoform analysis.
dc.languageEnglish
dc.subject.classificationbic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSB Biochemistry
dc.subject.otherproteoforms, top-down mass spectrometry, therapeutic proteins, liquid chromatography, protein purification parameter screening, displacement chromatography
dc.titleChapter Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry
dc.typechapter
oapen.identifier.doi10.5772/intechopen.89644
oapen.relation.isPublishedBy09f6769d-48ed-467d-b150-4cf2680656a1
oapen.relation.isFundedByH2020-MSCA-ITN-2017
oapen.grant.number765502
oapen.grant.acronymA4B


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