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dc.contributor.editorMaiato, Helder
dc.date.accessioned2022-06-09T10:06:48Z
dc.date.available2022-06-09T10:06:48Z
dc.date.issued2020
dc.identifier.urihttps://library.oapen.org/handle/20.500.12657/56692
dc.description.abstractThis volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.en_US
dc.languageEnglishen_US
dc.relation.ispartofseriesMethods in Molecular Biologyen_US
dc.subject.othermicrotubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migrationen_US
dc.titleCytoskeleton Dynamicsen_US
dc.title.alternativeMethods and Protocolsen_US
dc.typebook
oapen.identifier.doi10.1007/978-1-0716-0219-5en_US
oapen.relation.isPublishedBy8cf70738-d164-4fbe-bf04-ca83ba19c992en_US
oapen.relation.hasChapter967025c8-0d95-4556-84c3-54acb283e936
oapen.relation.isbn9781071602188en_US
oapen.relation.isbn9781071602218en_US
oapen.relation.isbn9781071602195en_US
oapen.series.number1en_US
oapen.pages390en_US


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