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    Chapter 48 In Vivo Bioluminescence Imaging to Assess Compound Efficacy Against Trypanosoma brucei

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    Author(s)
    Ritchie, Ryan
    Barrett, Michael
    Mottram, Jeremy
    Myburgh, Elmarie
    Collection
    Wellcome
    Language
    English
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    Abstract
    Traditional animal models for human African trypanosomiasis rely on detecting Trypanosoma brucei brucei parasitemia in the blood. Testing the efficacy of new compounds in these models is cumbersome because it may take several months after treatment before surviving parasites become detectable in the blood. To expedite compound screening, we have used a Trypanosoma brucei brucei GVR35 strain expressing red-shifted firefly luciferase to monitor parasite distribution in infected mice through noninvasive wholebody bioluminescence imaging. This protocol describes the infection and in vivo bioluminescence imaging of mice to assess compound efficacy against T. brucei during the two characteristic stages of disease, the hemolymphatic phase (stage 1) and the encephalitic or central nervous system phase (stage 2).
    Book
    Trypanosomatids
    URI
    https://library.oapen.org/handle/20.500.12657/47835
    Keywords
    Firefly luciferase, Trypanosoma brucei brucei, In vivo imaging, Bioluminescence
    DOI
    10.1007/978-1-0716-0294-2_48
    ISBN
    9781071602935, 9781071602966, 9781071602942
    Publisher
    Springer Nature
    Publisher website
    https://www.springernature.com/gp/products/books
    Publication date and place
    2020
    Grantor
    • Wellcome Trust - 104976,104111
    Classification
    Biology, life sciences
    Pages
    17
    Rights
    https://creativecommons.org/licenses/by/4.0/
    • Imported or submitted locally

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    License

    • If not noted otherwise all contents are available under Attribution 4.0 International (CC BY 4.0)

    Credits

    • logo EU
    • This project received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 683680, 810640, 871069 and 964352.

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